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HALL OF SCIENCE INSTRUMENTATION CENTER

The laboratories at Whitman College are well equipped with standard equipment including water baths, pH meters, balances, stir plates, Spectronic-20 colorimeters, etc. A number of specialized scientific instruments are also available for use in teaching and student research.


Chromatography

Chromatography is at the heart of analytical chemistry and environmental chemistry. While many types of instrumentation are designed to analyze relatively pure samples, chromatography systems are designed to analyze complex sample matrices (samples containing more than one analyte). We have a number of liquid and gaseous chromatographic systems for use in the classroom and in senior research. These instruments are listed below.

Hewlett-Packard 5890 Series II Gas Chromatograph / Hewlett-Packard 5971A Mass Spectral Analyzer

This instrument combines the superior separation power of capillary column GC with the confirmatory identification ability of mass spectrometer. This instrument is primary used in second-year organic chemistry laboratory. Purchased with funding from the NSF.

 

Agilent 6890N-FID/ECD Capillary Column Gas Chromatograph

This system is equipped with an autosampler, capillary split/splitless injector, and flame ionization and electron capture detectors. Peak area and height measurements are completed with a computer control station. Its principle use is the quantitative measurement of hydrocarbon mixtures in water, sediment/soil, and gaseous samples. The instrument is used in instrumental analysis and environmental chemistry labs, as well as student research. Purchased with funding for the Alden Foundation.

 

Agilent 6890 Capillary Column Gas Chromatograph/5973N Mass Spectrometer

This is our analytical/environmental GC-MS used to conduct research and teach instrumental analysis and mass spectrometer. It is equipped with an autosampler and EI and CI ionization systems. Purchased with funding from the M.J. Murdock Charitable Trust, Whitman College, and Whitman Alumni.

 

Hewlett-Packard 5890 FID Capillary Column Gas Chromatograph

This system is equipped with a capillary split/splitless injector and flame ionization detector. Peak area and height measurements are completed on a Hewlett-Packard 3396A integrator. Its principle use is the quantitative measurement of hydrocarbon mixtures in water, sediment/soil, and gaseous samples. The instrument is used in instrumental analysis and environmental chemistry labs, as well as student research. Donation for Environmental Protection Agency.

 

Two Shimadzu 2014 Capillary Column Gas Chromatographs

These systems are used to introduce students to chromatography in our advanced general chemistry course.

 

Shimadzu GC-14A Packed Column Gas Chromatograph

For use in polymer characterization and surface chemistry research. Purchased with internal funding.

 

Varian 3700 Packed Column Gas Chromatograph

This is a packed column gas chromatograph and is used in organic laboratory to determine product yield. It is equipped with a thermoconductivity detector, a flame ionization detector, and a 63-Ni electron-capture detector. Peak integration is completed with a Hewlett-Packard 3396 series II integrator.

Dionex DX-500 High Performance Liquid Chromatograph

The Dionex DX-500 High Performance Liquid Chromatography (HPLC) System consists of an automatic injection system, gradient pump and proportioning valve, UV-Vis spectrophotometer, and a Dell data processing station. This instrument is primary used in instrumental analysis and biochemistry research. Purchased with internal funding.

Dionex DX-300 Ion Chromatograph

Our Dionex DX-300 Ion Chromatography System is capable of analyzing inorganic cations and anions. Peak analysis is performed on a Hewlett-Packard 3396A integrator. This instrument is used in instrumental analysis and environmental chemistry, as well as in senior research. Donation from the US Department of Energy.

Dionex/HP High Performance Liquid Chromatograph

The Dionex DX-300 HPLC System is equipped with UV-Vis spectrophotometer and fluorescence detectors. Peak integration is performed with a Hewlett-Packard 3396A integrator. This instrument is primarily used in instrumental analysis and senior research. Donation from the US Department of Energy.

Pharmacia Fast Protein Liquid Chromatograph

The FPLC used for protein purification for structural biology and protein biochemistry.


Spectroscopy

There are many forms of atomic and molecular spectroscopy and we have a variety of instruments in our labs. We are currently seeking expansion of our NMR instruments, with a request to the NSF for a 400 MHz NMR.

Perkin-Elmer 1100B Flame Atomic Absorption Spectrometer

The Perkin-Elmer 1100B FAAS is an instrument for analyzing trace metal concentrations in water. It is equipped with a air-acetylene flame burner and can measure part-per-million levels for most metals. This instrument is used in research, instrumental analysis and environmental chemistry. Donation for the US Department of Energy.

Perkin-Elmer 3030B Flame/Graphite Furnace Atomic Absorption Spectrometer

Our Perkin-Elmer 3030B Atomic Absorption Spectrometer / Graphite Furnace is used in ultra-low trace metal analysis. Typical detection limits are in the parts-per-billion range. This instrument is used in research, instrumental analysis and environmental chemistry. Donation for the US Department of Energy.

 

Agilent 7500ce ICP-MS

This instrument is used to measure isotopic concentrations in aqueous samples or digested solid samples. It is used in research and instrumental methods of analysis. Purchased with funding from the M.J. Murdock Charitable Trust, the Camille and Henry Dreyfus Foundation, Whitman College, and Whitman Alumni.

 

Jasco V-550 UV-Vis Spectrophotometer

The Jasco V-550 UV-Vis Spectrophotometer is a basic scanning UV-Vis system, but with temperature control of the cell. It is used in biochemistry, physical chemistry, instrumental analysis, and environmental chemistry. Purchased with internal funding.

 

Jasco FP-6200 Fluorescense Spectrophotometer

The fluorescense spectrophotometer is a basic scanning system, but with temperature control of the cell. It is used in biochemistry, physical chemistry, instrumental analysis, and environmental chemistry. Purchased with internal funding.

 

Thermo Nicolet Avatar 360 FT-IR

This instrument is a workhorse of organic chemistry where students look for the presence of specific functional groups in an effort to identify synthesis products. Purchased with internal funding.

 

Jasco J-600 Spectropolarimeter

This instrument is used to measure circular dichroism typically on protein samples in biophysics lab classes and for research in structural biology. Includes a CD250 Peltier temperature controller from Quantum Northwest. Purchased with internal funding.


Bruker Avance III 400 MHz Ultrashield Plus Spectrometer System


The 400 MHz NMR includes a broadband probe tunable from 15N to 19F, with 1H decoupling and a Z-gradient coil, an inverse broadband probe that allows for maximum 1H-observe sensitivity with decoupling that covers the range from 109Ag to 31P and a Z-gradient coil, a variable temperature unit and a 60-slot sample changer. The primary use of the instrument is to characterize the structure and interactions of complex molecules, ranging from simple 1D experiments for 1H and 13C nuclei to 2D experiments such as DQF-COSY, TOCSY, NOESY, HSQC, HMQC, and HMBC. This instrument was installed in January 2010 and is used in organic, inorganic, and physical chemistry labs as well as undergraduate research.

 


Misc. Equipment

 

Oxford Diffraction Xcalibur Nova X-ray Crystallography System

This instrument will be used in courses and research for physics, chemistry, geology and BBMB (biochemistry, biophysics and molecular biology). The system includes a Cu-K alpha sealed tube X-ray source, focusing optics, two-dimensional ccd (charged-couple device) detector and a cryogenic cooling system for temperature control of the sample. X-ray diffraction data can be collected on both single crystals (both small molecule and protein) and powders. Faculty and students will use the system to analyze a wide variety of samples, including protein crystals, small molecule crystals, mixed amorphous and crystalline polyethylene, metal doped sol-gel glasses, liquids and mineral powders. Purchased with funding for the NSF.

 

 

Agilent G1601A Capillary Electrophoresis System

This instrument is used to separate a variety of inorganic and organic compounds ranging from chloride ions to large biomolecules. Purchased with funding from the M.J. Murdock Charitable Trust, Whitman College, and Whitman Alumni.

 

Shimadzu DSX-60 Differential Scanning Calorimeter


The DSC analyzes the heat associated with phase transitions of microgram quantities of substances from temperatures of -150C to 500C. It provides the tools by which we can determine the enthalpies and characteristic temperatures for these transitions in polymeric materials. It also provides a means by which we can determine the degree of crystallinity in semi-crystalline polymers. Purchased with internal funding.

 

 

Dohrmann Phoenix 8000 UV-Persulfate TOC Analyzer

This instrument, when set up, will be used to measure total organic carbon concentrations in aqueous samples.

 

 

Plas-Labs Model 855-AC Controlled Atmosphere Chamber

This chamber, commonly called a glove box, is used for work in oxygen-free situations and for critical isolation of either the operator or the research materials. It is used primarily in the advanced laboratory coursework and in senior research.

 

 

Shimadzu Thermogravimetric Analyzer TGA-50

The TGA analyzes the mass of a sample as a function of temperature from room temperature to a temperature of 1000C. This method allows us to perform burn-off analysis of residual chemicals on mineral additives. It also permits the determination of waters of hydration and weight gain arising from oxidation of a material. Purchased with internal funding.

 

 

EG&G Princeton Applied Research Model 264 Polarographic Analyzer/Stripping Voltammeter with Model 303A Static Mercury Drop Electrode

This instrument is occasionally used to teach voltammetery in our analytical chemistry course.

 

 

Parr Adiabatic Calorimeter

The Parr Adiabatic Calorimeter is used in physical and polymer chemistry to determine energy content of pure compounds.

 

Field Equipment


A variety of field equipment is available for use in environmental chemistry and in student research. This equipment is primarily used in the analysis of stream samples.


Soil corer

Van Dorn Water sampler

Hach and Orion pH meters

Orion selective ion probes (Chloride, Bromide, Fluoride, Nitrate, Nitrite, and Calcium)

Hach Conductivity meter

Orion dissolved oxygen (DO) probe with a 10-meter cable

Turbidity meter

Hach DR2010 spectrophotometer

Hach model 16900 field titration unit

Hach water analyst kit

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